Chlorpyrifos and its metabolites alter gene expression at non-cytotoxic concentrations in D3 mouse embryonic stem cells under in vitro differentiation: Considerations for embryotoxic risk assessment

Article ID	Journal	Published Year	Pages	File Type
5860565	Toxicology Letters	2013	9 Pages	PDF

Abstract

âº Chlorpyrifos and its metabolites alter gene expression in mouse embryonic stem cells. âº Chlorpyrifos and its metabolites alter gene expression at non-cytotoxic concentrations. âº Chlorpyrifos and its metabolites alter pluripotency of mouse embryonic stem cells. âº Chlorpyrifos and its metabolites alter differentiation of three embryonal lineages. âº Chlorpyrifos might alter differentiation without detectable maternal toxicity.

Keywords

Nanog homeobox CPF TLV DEPC qRT-PCR NTE NANOG ECx IC50 TCLP CPO Pnpla6 FLK1 DMEM nES LIF phenyl valerate PBS 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide 3,5,6-Trichloro-2-pyridinol Dulbecco's modified Eagle's medium MTT α-fetoprotein Embryonic stem cell test AChE Risk assessment EST Organophosphorus compound AFP Differentiation diethylpyrocarbonate Myosin heavy chain Embryonic stem cell Phosphate buffered saline MHC Threshold limit value nestin neuropathy target esterase quantitative real-time PCR Chlorpyrifos-oxon Chlorpyrifos