Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5860585 | Toxicology Letters | 2013 | 9 Pages |
Abstract
The blood-retinal barrier (BRB) is critical for maintaining retina homeostasis and low permeability. In this study, we evaluated the effects of electromagnetic pulse (EMP) exposure on the permeability of BRB, alterations of tight junction (TJ) proteins of BRB and if any, involvement of mitogen-activated protein kinase (MAPK) pathway. Male Sprague-Dawley (SD) rats and RF/6A cells which were pretreated with or without MAPKs inhibitors were sham exposed or exposed to EMP at 200Â kV/m for 200 pulses. The alteration of BRB permeability was examined through fluorescence microscope and quantitatively assessed using Evans blue (EB) and endogenous albumin as tracers. The expressions of TJ proteins and some signaling molecules of MAPK pathway were measured by Western blots. The observations were that EMP exposure resulted in increased BRB permeability concurrent with the decreased expressions of occludin and claudin-5, which were correlated with the increased expressions of phospho-p38, phospho-JNK and phospho-ERK and could be blocked when pretreated with p38 MAPK inhibitor. Thus, the results suggested that the alterations of occludin and claudin-5 may play an important role in the disruption of TJs, which may lead to the transient breakdown of BRB after EMP exposure with the involvement of p38 MAPK pathway through phosphorylation of signaling molecules.
Keywords
SDSTris buffered saline with Tween 20BRBTBSTBCAEMPGAPDHPVDFFBSMAPKTight junctionEvans Bluebicinchoninic acidpolyvinylidene difluoridesodium dodecyl sulfatefetal bovine serumblood–retinal barrierPermeabilityElectromagnetic pulseTight junction proteinmitogen-activated protein kinaseGlyceraldehyde phosphate dehydrogenase
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Authors
Hai-Juan Li, Liang-Mei Guo, Long-Long Yang, Yong-Chun Zhou, Yan-Jun Zhang, Juan Guo, Xue-Jun Xie, Guo-Zhen Guo,