Effect of serum on diesel exhaust particles (DEP)-induced apoptosis of airway epithelial cells in vitro

Article ID	Journal	Published Year	Pages	File Type
5860633	Toxicology Letters	2013	9 Pages	PDF

Abstract

âº Serum modulates effects of DEP on airway epithelial cell death. âº Serum modulates effects of DEP on inflammation of airway epithelial cells. âº Serum makes airway epithelial cells more susceptible to deleterious effects of DEP. âº This involves oxidant stress pathways such as JNK and NF-ÎºB.

Keywords

GM-CSF DEP O2-ERK sICAM-1 NAC Cyclin-dependant kinase BEC PM10 regulated on activation, normal T-cell expressed and secreted Jnk TGF DMEM FCS FACS CDK NF-κB 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide DMSO Dulbecco's modified Eagle Medium MTT N-acetylcysteine Hydrogen peroxide inflammation interleukin Interleukin-8 Chronic obstructive pulmonary disease COPD transforming growth factor dimethyl sulphoxide Diesel exhaust particles Cell viability Serum foetal calf serum Bronchial epithelial cells Nuclear factor-kB fluorescence-activated cell sorter granulocyte macrophage-colony stimulating factor RANTES soluble intercellular adhesion molecule 1 H2O2 activator protein Propidium iodide extracellular signal-regulated kinase