| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5860686 | Toxicology Letters | 2012 | 9 Pages |
Abstract
⺠UGT1A6 and UGT1A7 expression and 1-naphthol enzymatic activity were analyzed in rat astrocytes. ⺠Cells were treated with xenobiotic compounds known to generate oxidative stress. ⺠The short term exposure inhibited the 1-naphthol glucuronidation as a result of protein oxidation. ⺠Oxidative stress induced deleterious changes in astrocyte morphology and decreased cell viability. ⺠Significantly higher glucuronidation activity and mRNA expression of detoxification enzymes in surviving impaired astrocytes.
Keywords
NADPH:cytochrome P450 reductase1-naphtholUDPGAFITCUGTsGFAPNrf2NQO1HBSSRT-PCRDMSONAD(P)H:quinone oxidoreductase 11-NPROSUDP-glucuronosyltransferasesUDP-glucuronosyltransferaseAstrocytesUDP-glucuronic acidOxidative stressDimethyl sulfoxydeHank's balanced saline solutionantioxidant response elementfluorescein isothiocyanatelactate dehydrogenaseLDHAREreverse transcription polymerase chain reactionParaquatGlial fibrillary acidic proteinReactive oxygen species
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Authors
Daniela Gradinaru, Anne-Laure Minn, Yves Artur, Alain Minn, Jean-Marie Heydel,