A decrease of intracellular ATP is compensated by increased respiration and acidification at sub-lethal parathion concentrations in murine embryonic neuronal cells: Measurements in metabolic cell-culture chips

Article ID	Journal	Published Year	Pages	File Type
5860866	Toxicology Letters	2011	9 Pages	PDF

Abstract

âº In vitro test strategy for cell-metabolic effects of toxins with murine embryonic, primary cortex cells (MEPCs). âº Cell-chips were used for the on-line registration of the parathion effect on respiration, acidification and cell adhesion. âº IC50 values were 100Â Î¼M (respiration), 65Â Î¼M (acidification), 54Â Î¼M (adhesion), and 33Â Î¼M (intracellular ATP-level). âº While ATP-level and cell adhesion showed a simple logistic decay, respiration and acidification were increased at low doses. âº This is a result of cellular regulation induced by the uncoupling of cellular respiration by parathion and its metabolites.

Keywords

PBS MM+Acetylcholine-esterase mEPC ISFET PDL MMT IC50 DMEM FBS P/S AChE gamma amino butyric acid standard deviation IdeS ion-selective field-effect transistor DIV days in vitro fetal bovine serum Neurotoxicity Metabolism culture medium Hormesis Poly-d-lysine Penicillin/streptomycin GABA