Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5861371 | Toxicology in Vitro | 2015 | 9 Pages |
Abstract
Few studies have addressed the cellular effects of bisphenol S (BPS) and bisphenol AF (BPAF) on cells, and no study has been conducted to analyze the mechanism of action of bisphenols in blood cells. In this study, the effect of bisphenol A (BPA), bisphenol F (BPF), BPS and BPAF on human peripheral blood mononuclear cells (PBMCs) was analyzed. It was shown that BPA, BPF and BPAF in particular, decreased cell viability, which was associated with depletion of intracellular ATP level and alterations in PBMCs size and granulation. Bisphenols enhanced ROS (including OH) formation, which led to damage to lipids and proteins in PBMCs. The most significant alterations in ROS level were induced by BPF, and particularly BPAF. Moreover, it was shown that BPAF most strongly provoked lipid peroxidation, while BPA and BPS caused the greatest damage to proteins. It may be concluded that BPA and its analogs were capable of inducing oxidative stress and damage in PBMCs in the concentrations ranging from 0.06 to 0.5 μM (0.02-0.1 μg/ml), which may be present in human blood as a result of environmental exposure. Although, most of bisphenols studied decreased cell viability, size and ATP level at higher concentrations, BPAF exhibited its cytotoxic potential at low concentrations ranging from 0.3 to 3 μM (0.1-1.0 μg/ml) that may correspond to concentrations in humans following occupational exposure.
Keywords
BPAPNACFSC2,4,5-TCPSSCPCP6-carboxy-2′,7′-dichlorodihydrofluorescein diacetatehpfBPFPBMCsBPAFBPS2,4,5-Trichlorophenolcis-parinaric acidBisphenolsBisphenol AFBisphenol Fbisphenol SBisphenol AMorphological alterationsCell viabilityperipheral blood mononuclear cellsLipid peroxidationPentachlorophenolReactive oxygen species
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Jaromir MichaÅowicz, Katarzyna Mokra, Agata BÄ
k,