Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5861695 | Toxicology in Vitro | 2014 | 10 Pages |
Abstract
In this study, we evaluated the size-dependent effects of AgNPs by treating the human LoVo cell line, an intestinal epithelium model, with spherical AgNPs of well-defined sizes (10, 20, 40, 60 and 100Â nm). The cellular uptake was visualized by confocal laser scanning microscopy, and various cytotoxicity parameters were analyzed in a size- and dose-dependent manner. In addition, the cellular proteomic response to 20 and 100Â nm AgNPs was investigated to increase the understanding of potential mechanisms of action. Our data indicated that cellular uptake and toxicity were regulated by size; smaller particles easily penetrated the cells, and 100Â nm particles did not. It was hypothesized that this size-dependent effect resulted from the stimulation of a signaling cascade that generated ROS and inflammatory markers, leading to mitochondrial dysfunction and subsequently inducing apoptosis. By contrast, the cell proliferation, was independent of AgNPs particle size, indicating a differentially regulated, ROS-independent pathway.
Keywords
AgNPIL-8DCFDAFITCCLSMPVDFSUMO2′,7′-dichlorofluorescein diacetate5-bromo-2′-deoxyuridineROSSEM–EDXTemInterleukin 8BrdUEnzyme-linked immunosorbent assayELISACellular uptakeCytotoxicityfluorescein isothiocyanateTransmission electron microscopyconfocal laser scanning microscopySilver nanoparticleSilver nanoparticlesGene ontologyPropidium iodidePolyvinylidene fluoridesmall ubiquitin-like modifierReactive oxygen species
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Authors
Rona Miethling-Graff, Rita Rumpker, Madeleine Richter, Thiago Verano-Braga, Frank Kjeldsen, Jonathan Brewer, James Hoyland, Horst-Günter Rubahn, Helmut Erdmann,