Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5861734 | Toxicology in Vitro | 2013 | 9 Pages |
Abstract
The ERα/ERβ ratio in T47D-ERβ cells exposed to >150 ng/ml tetracycline is comparable to the ratio found in rat mammary gland and in human breast tissue. The ERα/ERβ ratio of other estrogen-sensitive rat and human tissues can also be mimicked in T47D-ERβ cells. The ERα/ERβ ratio found in MCF-7 and native T47D breast cancer cell lines did not reflect ratios in analyzed rat and human tissues, which further supports the use of T47D-ERβ cells as model for estrogen-responsive tissues. Using 17β-estradiol and the T47D-ERβ cells under the conditions defined to mimic various tissues it could be demonstrated how these different tissues vary in their proliferative response.
Keywords
qPCRHE stainingEC50dNTPMSDHP1mRNAPVDFIgGSDSDMEMT47D cellsFCSPBSM-MLVDCC-FCScDNADNAEDTAEthylenediaminetetraacetic aciddeoxyribonucleic acidcomplementary deoxyribonucleic acidimmunoglobulin GBreast tissueReverse transcriptaseDetection limitdeoxynucleotide triphosphatemessenger ribonucleic acidhematoxylin and eosin stainingsodium dodecyl sulfatefetal calf serumMCF-7 cellshuman breast adenocarcinoma cellsPhosphate buffered salineDulbecco’s modified eagle’s mediumhalf maximal effective concentrationBenign prostatic hyperplasiaBPHquantitative polymerase chain reactionMoloney murine leukemia virusheterochromatin protein 1Estrogen receptorestrogen receptors
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Authors
N.M. Evers, T.M.C. van de Klundert, Y.M. van Aesch, S. Wang, W.K. de Roos, A. Romano, L.H.J. de Haan, A.J. Murk, A.G.H. Ederveen, I.M.C.M. Rietjens, J.P. Groten,