Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5861748 | Toxicology in Vitro | 2013 | 7 Pages |
Abstract
Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.
Keywords
FADDFas associated death domainapoptotic protease-activating factorDestruxin BAPAFJC-1FITCFACS5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodideAIFApoptosisfluorescence-activated cell sortingapoptosis-inducing factorfluorescein isothiocyanateNon-Hodgkin lymphomaDeath receptor pathwayMitochondrial membrane potentialPropidium iodide
Related Topics
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Environmental Science
Health, Toxicology and Mutagenesis
Authors
Pin-Zhir Chao, Yi-Ping Chin, I-Uen Hsu, Cheng-Ming Liu, Ya-Chi Yu, Ting-Kai Leung, Yuarn-Jang Lee, Chien-Ho Chen, Yung-Feng Lin,