Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5861790 | Toxicology in Vitro | 2013 | 9 Pages |
Abstract
A reactive oxygen species (ROS) assay was previously developed for photosafety assessment; however, the phototoxic potential of some chemicals cannot be evaluated because of their limited aqueous solubility. The present study was undertaken to develop a new micellar ROS (mROS) assay system for poorly water-soluble chemicals using a micellar solution of 0.5% (v/v) Tween 20 for solubility enhancement. In repeated mROS assay, intra- and inter-day precisions (coefficient of variation) were found to be below 11%, and the Zâ²-factors for singlet oxygen and superoxide suggested a large separation band between positive and negative standards. The ROS and mROS assays were applied to 65 phototoxins and 18 non-phototoxic compounds for comparative purposes. Of all 83 chemicals, 25 were unevaluable in the ROS assay due to poor solubility, but only 2 were in the mROS assay. Upon mROS assay on these model chemicals, the individual specificity was 76.5%, and the positive and negative predictivities were found to be 93.9% and 86.7%, respectively. The mROS assay provided 2 false negative predictions, although negative predictivity for the ROS assay was found to be 100%. Considering the pros and cons of these assays, strategic combined use of the ROS and mROS assays might be efficacious for reliable photosafety assessment with high applicability and predictivity.
Keywords
SDSNaPBOECDNBTDMSOP-aminobenzoic acidROSnitroblue tetrazoliumUltravioletstandard deviationsodium phosphate bufferDimethyl sulfoxideMicellar solutionthe Organisation for Economic Co-operation and Developmentsodium dodecyl sulfateCoefficient of VariationPhotoreactivityPhototoxicityPABAReactive oxygen species
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Authors
Yoshiki Seto, Masashi Kato, Shizuo Yamada, Satomi Onoue,