Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5861903 | Toxicology in Vitro | 2015 | 5 Pages |
Abstract
Tributyltin and triphenyltin are known as endocrine disruptors. Moreover, the compounds exert their toxicity in mammals predominantly through nuclear receptor signaling. Here we present the effects of tributyltin chloride (TBT-Cl) and triphenyltin chloride (TPT-Cl) on cell proliferation, expression of proapoptotic p53, Bax, and antiapoptotic Bcl-2 proteins in human breast cancer MCF-7 cell line. Dose and time dependent (24, 48 and 72Â h) cell expositions have demonstrated TBT-Cl as more effective in inhibiting MCF-7 cell proliferation than TPT-Cl. Short time treatment with TBT-Cl displayed marked stimulation of p53 protein expression when compared to TPT-Cl. Both organotin compounds displayed similar mild enhancement of Bax protein expression. The 24Â h exposition of TPT-Cl induced substantial diminution of Bcl-2 protein expression in comparison with both, untreated cells and TBT-Cl treated cells. Our observations indicate that TBT-Cl and TPT-Cl have different antiproliferative potency and distinct impact on expression of apoptosis marker proteins.
Keywords
JnkHuman breast carcinoma cellsTributyltin chlorideRXRRARERKMCF-7FBSatRAc-Jun N-terminal kinaseMAPKall-trans retinoic acidAntiproliferative effectsTriphenyltin chlorideOrganotin compoundsApoptosisfetal bovine serumhuman breast cancer cell linemitogen-activated protein kinaseextracellular-signal-regulated kinase
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Authors
Maria Fickova, Ladislav Macho, Julius Brtko,