A dioxin response element in the multiple cloning site of the pGL3 luciferase reporter influences transcriptional activity

Article ID	Journal	Published Year	Pages	File Type
5862144	Toxicology in Vitro	2012	6 Pages	PDF

Abstract

âº Dioxin response element identified in multiple cloning site of pGL3 luciferase vector. âº Multiple cloning site dioxin response element induces transcriptional activity. âº Effects are selective to dioxin sensitive promoter and enhancer elements.

Keywords

PBS DRE ARNT AP-1 TCDD NF-κB LPS AHR 2,3,7,8-Tetrachlorodibenzo-p-dioxin DMSO Oct immunoglobulin Luciferase reporter Dimethyl sulfoxide dioxin response element nuclear factor-κB AhR nuclear translocator Phosphate buffered saline Transcriptional activity lipopolysaccharide activator protein 1 aryl hydrocarbon receptor