Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5862274 | Toxicology in Vitro | 2014 | 10 Pages |
Abstract
However, 6-α, 6-β and 16-β-hydroxytestosterone were produced about 20-fold less as compared to androstenedione and the production of metabolites depended on the culture media supplemented with 2% mouse serum or 10% FCS. Finally, by RT-PCR expression of CYP genes was confirmed in lung tissue and AECs; a link between testosterone metabolism and CYP2A12, 3A16 and 2B9/10 expression was established. Taken collectively, AECs can be successfully isolated and cultured for six days while retaining metabolic competence.
Keywords
ERODA/J miceNNKFMOAECsFCSethoxyresorufin-O-deethylasei.p.NBTDMEMPAHFITCHEPESGSHCyP4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidB[a]PDulbecco’s modified Eagle mediumAlkaline phosphataseSprague DawleyBenzo[a]pyreneGene expressionnitro blue tetrazoliumintraperitonealfetal calf serumalveolar epithelial cellsCytochrome P450 monooxygenasescytochrome Pfluorescein isothiocyanatePolycyclic aromatic hydrocarbonhigh-performance liquid chromatographyHPLCglutathione S-transferase
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Authors
Tanja Hansen, Anil Chougule, Jürgen Borlak,