Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells

Article ID	Journal	Published Year	Pages	File Type
5862339	Toxicology in Vitro	2012	9 Pages	PDF

Abstract

âº Different-coated cell culture dishes did not enhance attachment of colon cells. âº Intestinal stem cells could be detected in the primary colon cell culture. âº Colon crypts prolong the lifespan of human intestinal epithelial cells up to 24Â h.

Keywords

DAB FCS SFs HBSS DTT EGF EDTA Ethylenediaminetetraacetic acid Cell isolation minimal essential medium diaminobenzidine dithiothreitol RIN fetal calf serum intestinal stem cells RNA Integrity Number epidermal growth factor Primary cell culture MEM