Development of an in vitro skin sensitization test based on ROS production in THP-1 cells

Article ID	Journal	Published Year	Pages	File Type
5862545	Toxicology in Vitro	2013	7 Pages	PDF

Abstract

âº A new in vitro skin sensitization test based on the production of ROS was developed. âº 30-min exposure to skin sensitizers induced ROS production in THP-1 cells. âº High predictivity on an in vivo test (82% accuracy) was obtained. âº Skin sensitization potency can be predicted based on ROS production.

Keywords

DCs Tetramethylthiuram disulfide TmTD BKC LLNA h-CLAT ECVAM DNFB TrxR PPD OXA DNCB HBSS DEP MFI HCA p-phenylenediamine 1-fluoro-2,4-dinitrobenzene 2,4-dinitrochlorobenzene DMSO ROS human cell line activation test Local lymph node assay Phthalic anhydride Octanoic acid Lactic acid Oxazolone Benzalkonium chloride thioredoxin reductase In vitro testing Animal testing alternatives Skin sensitization Diethyl phthalate Dimethylsulfoxide THP-1 cells Dendritic cells Methyl salicylate Hank’s balanced salt solution European Centre for the Validation of Alternative Methods mean fluorescence intensity Propidium iodide propyl gallate Chlorobenzene Reactive oxygen species (ROS)Reactive oxygen species