DIESEL particulate exposed macrophages alter endothelial cell expression of eNOS, iNOS, MCP1, and glutathione synthesis genes

Article ID	Journal	Published Year	Pages	File Type
5862741	Toxicology in Vitro	2011	10 Pages	PDF

Abstract

âº Diesel exhaust particulate (DEP) has been suggested to traverse alveolar barriers. âº But, DEP vascular effects may be related to the release of inflammatory cytokines. âº We found that direct DEP exposure to endothelium perturbed cell function. âº Endothelial responses were greater when co-cultured with DEP exposed macrophages. âº DEP vascular effects are likely due to release of soluble factors by macrophages.

Keywords

PM2.5 iNOS DEP RT-PCR GSH MCP1 AREs BH4 tetrahydrobiopterin IL6 GCLC FBS GCLM eNOS ECE1 TNFα PBS GCL GMCSF 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide DMSO IL1β MTT ONOO−ROS inflammation endothelin endothelin-1 interleukin 1β interleukin 6 analysis of variance ANOVA tumor necrosis factor α standard error of the mean Dimethylsulfoxide Diesel exhaust particulate particulate matter fetal bovine serum inducible nitric oxide synthase endothelial nitric oxide synthase Granulocyte macrophage colony stimulating factor antioxidant response elements Phosphate buffered saline glutamate cysteine ligase SEM Noxs Nitric oxide nitric oxide synthase monocyte chemoattractant protein 1 Peroxynitrite glutamate cysteine ligase modifier subunit Glutamate cysteine ligase catalytic subunit Glutathione Reactive oxygen species