Comparison of a genomic and a multiplex cell imaging approach for the detection of phospholipidosis

► The comparison of phospholipidosis detection with the use of HepG2 cells (in vitro) and Wistar rats (in vivo). ► In vitro, the imaging fluorescent method is preferred over the genomic method because it is more adapted to medium throughput. ► In vivo, findings of lung foamy macrophages and vacuolization correlates more with the fluorescent than with the genomic method.