Exocytosis of MTT formazan could exacerbate cell injury

Article ID	Journal	Published Year	Pages	File Type
5862975	Toxicology in Vitro	2012	9 Pages	PDF

Abstract

âº We test the effect of MTT itself on in vitro SH-SY5Y cells. âº MTT can induce damages by not its endocytosis but its metabolism and exocytosis. âº MTT itself can activate caspase, injury cell membrane and cause content's leakage.

Keywords

DAPI PBS SH-SY5Y DMEM SDS Aβ PMSF CLSM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 4′,6-diamidino-2-phenylindole DMSO Dulbecco’s modified Eagle medium MTT Endocytosis EDTA Ethylenediaminetetraacetic acid Exocytosis Tem amyloid beta Dimethyl sulfoxide sodium dodecyl sulfate phenylmethyl sulfonylfluoride lactate dehydrogenase LDH Phosphate-buffered saline Cell death Confocal laser scanning microscope Transmission electron microscopy Propidium iodide