Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5888502 | Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) | 2013 | 9 Pages |
The goal of the present study was to determine whether endogenous synthesis of nâ3 polyunsaturated fatty acids (PUFA) in the fat-1 mouse is comparable to fish oil feeding with respect to kidney nâ3 PUFA composition and eicosanoid levels. Wild-type and heterozygous fat-1 mice, capable of synthesizing nâ3 PUFA endogenously, were given diets enriched in either nâ3 or nâ6 PUFA in a 2Ã2 factorial design and terminated after 12 weeks. Kidney phospholipid fatty acids were analysed by gas chromatography. Kidney eicosanoids were analysed by liquid chromatography tandem mass spectrometry. Relative to control mice fed nâ6 PUFA, nâ3 PUFA fed and fat-1 mice had higher levels of kidney phospholipid nâ3 PUFA, and lower levels of nâ6 PUFA and eicosanoids. However, mice fed nâ3 PUFA mice had higher levels of nâ3 PUFA and lower levels of eicosanoids as compared to fat-1 mice. In conclusion, diet feeding had a greater impact on kidney fatty acid composition and eicosanoid levels than the genetic effect of the fat-1 gene. However, the fat-1 mouse remains a close approximation that can be used as a complementary model to study the role of nâ3 PUFA in the kidney.