| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5894013 | Journal of Genetics and Genomics | 2016 | 12 Pages |
Abstract
The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants, and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing. Here, we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects. Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.
Keywords
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Developmental Biology
Authors
Dandan Zhang, Zhenxiang Li, Jian-Feng Li,