Article ID Journal Published Year Pages File Type
5896228 Placenta 2009 6 Pages PDF
Abstract
We report that different protocols are required for BeWo choriocarcinoma cells (nucleofection), primary cytotrophoblast cells (lipid-based transfection) and villous tissue explants (nucleofection). The results provide guidelines for optimal siRNA-mediated knockdown in these three models of the human placenta.
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Life Sciences Biochemistry, Genetics and Molecular Biology Developmental Biology
Preview
Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines
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