Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5896594 | Cytokine | 2016 | 8 Pages |
â¢First study to identify the distinctive immune factors from ocular surface of HIV.â¢Epithelial growth factor (EGF) levels were higher in tears of DED patients with HIV.â¢Interferon gamma-induced protein 10 (IP-10) levels were high.â¢Growth related oncogene (GRO) levels were lower in the tears of DED patients.
PurposeTo investigate the tear cytokine profile in HIV patients with dry eye disease (DED) and study the association between the severity of ocular inflammatory complications and tear cytokines levels. We postulate that HIV-mediated inflammation may be the underlying pathogenic mechanism for HIV-associated DED.MethodsThe current prospective case-control study compared tear film cytokine profiles in DED patients with HIV infection (n = 34) and age/gender-matched DED patients without HIV infection [controls (n = 32)]. Participants were recruited from tertiary referral eye care centre and communicable disease clinics, Singapore. Ocular surface health was documented using tear film, Schirmer's test, corneal staining, and conjunctival injection measurements. Tear samples were collected using Schirmer's strips and analysed for the levels of 41 cytokines using Luminex bead assay. Logistic regression models were performed to determine correlation and significance.ResultsAmong the 41 cytokines analysed, statistically significant differences were observed in the mean values of epithelial growth factor (EGF), growth related oncogene (GRO) and interferon gamma-induced protein 10 (IP-10). EGF and IP-10 levels were higher and GRO levels were lower in the tears of DED patients with HIV infection compared to DED patients without HIV infection. No significant association was found between varying levels of ocular surface parameters and cytokine concentrations in HIV patients with DED (p > 0.05).ConclusionsEGF and IP-10 were significantly elevated and GRO levels were lower in the tear profile of HIV patients with DED compared to immunocompetent patients with DED. This study suggests a novel cytokine driven paradigm for ocular inflammatory complications of HIV infection. Additional studies in large organised cohorts can validate the results.