Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5896654 | Cytokine | 2016 | 13 Pages |
Abstract
The innate immune response represents a primary line of defense against invading viral pathogens. Since epithelial cells are the primary site of gammaherpesvirus replication during infection in vivo and there are no information on activity of IFN-III signaling against gammaherpesviruses in this cell type, in present study, we evaluated the expression profile and virus-host interactions in mouse mammary epithelial cell (NMuMG) infected with three strains of murine gammaherpesvirus, MHV-68, MHV-72 and MHV-4556. Studying three strains of murine gammaherpesvirus, which differ in nucleotide sequence of some structural and non-structural genes, allowed us to compare the strain-dependent interactions with host organism. Our results clearly demonstrate that: (i) MHV-68, MHV-72 and MHV-4556 differentially interact with intracellular signaling and dysregulate IFN signal transduction; (ii) MHV-68, MHV-72 and MHV-4556 degrade type I IFN receptor in very early stages of infection (2-4Â hpi), but not type III IFN receptor; (iii) type III IFN signaling might play a key role in antiviral defense of epithelial cells in early stages of murine gammaherpesvirus replication; (iv) NMuMG cells are an appropriate model for study of not only type I IFN signaling, but also type III IFN signaling pathway. These findings are important for better understanding of individual virus-host interactions in lytic as well as in persistent gammaherpesvirus replication and help us to elucidate IFN-III function in early events of virus infection.
Keywords
VSVIRF-3MAVSInterferon type IHHV-8ISGMurine gammaherpesvirus 68RtaKSHVTBK1IRF-7PKRFBShpiMHV-68Interferon regulatory factor-7GAPDHIFNARJAK/STATMOIHuman Herpesvirus 8EBVinterferoninterferon lambdaIFNTANK binding kinase 1Kaposi’s sarcomahours post infectionfetal bovine serummitochondrial antiviral signalingInterferon regulatory factor 3Epstein-Barr virusVesicular stomatitis virusHuman herpesvirus 4Kaposi’s sarcoma-associated herpesvirusprotein kinase Rmultiplicity of infectioninterferon-stimulated genejanus kinase/signal transducers and activators of transcriptionglyceraldehyde 3-phosphate dehydrogenase
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Authors
KatarÃna LopuÅ¡ná, TÃmea Benkóczka, Jakub Lupták, Radka MatúÅ¡ková, ĽubomÃra LukáÄiková, Ingrid OveÄková, Ingeborg Režuchová,