Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5897082 | Cytokine | 2014 | 8 Pages |
Abstract
Interleukin-35 (IL-35), an IL-12 cytokine family member, mediates the immune inhibitory function of regulatory T cells (Treg). We assayed the presence of IL-35 in paraffin-embedded human pancreas cancer (PCAN) and unexpectedly found IL-35 was expressed mainly by epithelial derived PCAN cells, but not by Treg. We further examined the expression and effect of exogenous IL-35 in human PCAN cell lines and found IL-35 promoted growth and inhibited apoptosis in PCAN cell lines. IL-35 induced proliferation correlated with an increase in cyclin B, cyclin D, cdk2, and cdk4 and a decrease in p27 expression, while inhibition of apoptosis was associated with an increase in Bcl-2 and a decrease in TRAILR1. We conclude IL-35 is produced by PCAN in vivo and promotes PCAN cell line growth in vitro. These results might indicate an important new role for IL-35 as an autocrine growth factor in PCAN growth.
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Authors
Michael B. Nicholl, Chelsea L. Ledgewood, Xuhui Chen, Qian Bai, Chenglu Qin, Kathryn M. Cook, Elizabeth J. Herrick, Alberto Diaz-Arias, Bradley J. Moore, Yujiang Fang,