Differential activation of the inflammasome in THP-1 cells exposed to chrysotile asbestos and Libby “six-mix” amphiboles and subsequent activation of BEAS-2B cells

Article ID	Journal	Published Year	Pages	File Type
5897316	Cytokine	2012	13 Pages	PDF

Abstract

âº Chrysotile and Libby activate the inflammasome in THP-1 cells with release of IL-1Î². âº Long chrysotile fibers induce more IL-1Î² than relatively shorter Libby amphiboles. âº IL-1Î² released in conditioned medium triggers MAPK-IL-6 signaling in BEAS-2B cells. âº Addition of IL-1Ra has the potential to block the IL-1Î² signaling in BEAS-2B cells.

Keywords

MnSOD Manganese SOD IKKs GCLC IκB kinases Phorbol-12-myristate-13-acetate NLRP3 IL-1RAcP TRAF6 IL-1RA IL-1β LiB MYD88 DNPH COT ASC Tpl2 2,4-dinitrophenylhydrazine PMA MAPK kinase kinase Chrysotile asbestos Electrophoretic mobility shift assay IL-1 receptor antagonist Epithelium TIR Glass beads SOD Surface area EMSA یا electrophoretic mobility shift assay Superoxide dismutase TNF receptor-associated factor 6 IRAK Macrophage myeloid differentiation primary response gene 88 IL-1 receptor accessory protein apoptosis-associated speck-like protein containing a CARD CHRY Chrysotile