Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34

► The active region of porcine CSF-1 and full length CSF-1R have been cloned. ► Biological activity of porcine CSF-1 has been demonstrated. ► The cross species reactivity of IL-34 has been investigated. ► Both mouse and human IL-34 are biologically active on the pig CSF-1R. ► CSF-1 and IL-34 structure analysis of species-specific activity has been performed.