Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5897339 | Cytokine | 2012 | 10 Pages |
Abstract
⺠The ability of a whole blood assay to predict cytokine release in vivo was assessed. ⺠The assay correctly identified the hazard for several clinically relevant antibodies. ⺠Aqueous TGN1412 induced cytokine release in whole blood in vitro. ⺠Compared to a PBMC assay, we found low background and no false-positive results. ⺠Assay format selection must focus on target biology and in vivo relevant mechanisms.
Keywords
PBSQCsTNFmAbLLOQPBMCsTGN1412ULOQPEIMSDMonoclonal antibodyCytokine releaseinterferonIFNinterleukinupper limit of quantificationlower limit of quantificationstandard error of the meancluster of differentiationWhole bloodRoom temperatureperipheral blood mononuclear cellsCoefficient of Variationtumor necrosis factorfragment crystallizablePhosphate-buffered salineSEMquality control samplesPolyethylenimineMeso Scale Discovery
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Endocrinology
Authors
Babette Wolf, Hannah Morgan, Jennifer Krieg, Zaahira Gani, Adriana Milicov, Max Warncke, Frank Brennan, Stewart Jones, Jennifer Sims, Andrea Kiessling,