Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5897711 | Cytokine | 2013 | 5 Pages |
Abstract
Interleukin-12 is a potent activator and initiator of type-1 T cell development, and can be used as an adjuvant to bias for the development of vaccine-induced Th1 immune responses. During vaccination of MHC class I deficient beta-2 microglobulin knockout mice (β2Mâ/â) with an IL-12/αIL-4 Th1 biasing procedure, all of the mice died. None of the IL-12/αIL-4 treated wild type mice developed any noticeable complications. We hypothesized that NK cells may be activated by IL-12 treatment in these β2Mâ/â mice, leading to necrosis and eventual death. IL-12/αIL-4 treatment of β2Mâ/â mice resulted in increased NK cell numbers and activation status (IFN-γ+, CD69+). Finally, in vivo depletion of NK cells reversed the pathology induced by IL-12/αIL-4 treatment in β2M deficient mice. These results indicate that IL-12 combined with αIL-4 irreversibly activates NK cells leading to a disseminated inflammatory pathology and death in β2Mâ/â mice.
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Authors
Christopher S. Eickhoff, Anita R. Schnapp, John E. Sagartz, Daniel F. Hoft,