Interlaboratory reproducibility of female genital tract cytokine measurements by Luminex: Implications for microbicide safety studies

► Three laboratories measure cytokines in cervicovaginal lavage samples by Luminex. ► IFN-γ, IL-10, IL-17 and TNF are below detection in a majority of CVL samples. ► IL-1β, IL-6 and IL-8, but not IFN-α2, show good agreement in absolute measurements. ► IL-1β and IL-6 show good agreement in within-subject change after microbicide gel use. ► Cytokine measurement by Luminex has potential utility in microbicide safety studies.