Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5901145 | General and Comparative Endocrinology | 2014 | 9 Pages |
â¢Sea bass has orthologhe genes for ASIP1, ASIP2, AGRP1 and AGRP2.â¢All four melanocortin antagonist are expressed in the brain.â¢Hypothalamic AGRP1 and AGRP2 are up- and down-regulated by fasting, respectively.â¢Fasting had no effect on hypothalamic ASIP1 expression.â¢Hypothalamic ASIP2 expression is up-regulated by short term fasting.
The melanocortin system is one of the most complex hormonal systems in vertebrates. Atypically, the signaling of melanocortin receptors is regulated by the binding of endogenous antagonists, named agouti-signaling protein (ASIP) and agouti-related protein (AGRP). Teleost specific genome duplication (TSGD) rendered new gene copies in teleost fish and up to four different genes of the agouti family of peptides have been characterized. In this paper, molecular cloning was used to characterize mRNA of the agouti family of peptides in sea bass. Four different genes were identified: AGRP1, ASIP1, AGRP2 and ASIP2. The AGRP1 gene is mainly expressed in the brain whereas ASIP1 is mainly expressed in the ventral skin. Both ASIP2 and AGRP2 are expressed in the brain and the pineal gland but also in some peripheral tissues. Immunocytochemical studies demonstrated that AGRP1 is exclusively expressed within the lateral tuberal nucleus, the homologue of the mammalian arcuate nucleus in fish. Long-term fasting (8-29Â days) increased the hypothalamic expression of AGRP1 but depressed AGRP2 expression (15-29Â days). In contrast, the hypothalamic expression of ASIP2 was upregulated during short-term fasting suggesting that this peptide could be involved in the short term regulation of food intake in the sea bass.