Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5904066 | Neuropeptides | 2016 | 42 Pages |
Abstract
In this study, we investigated the effect of olanzapine on ghrelin-mediated GHS-R signaling by using an electrical impedance-based receptor biosensor assay system (CellKeyâ¢). Olanzapine at concentrations of 10â 7 and 10â 6 mol/L enhanced ghrelin-induced (10â 10-10â 8 mol/L) GHS-R activation. A Ca2 + imaging assay revealed that olanzapine (10â 7 and 10â 6 mol/L) enhanced ghrelin (10â 7 M)-induced GHS-R activity. In contrast, haloperidol (an antipsychotic agent) failed to enhance this ghrelin-mediated GHS-R activation, as demonstrated by both the CellKey⢠and Ca2 + imaging assays. Together, these results suggest that olanzapine, but not haloperidol, promotes appetite by enhancing ghrelin-mediated GHS-R signaling.
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Authors
Keita Tagami, Yohei Kashiwase, Akinobu Yokoyama, Hitomi Nishimura, Kanako Miyano, Masami Suzuki, Seiji Shiraishi, Motohiro Matoba, Yuichiro Ohe, Yasuhito Uezono,