Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905142 | Gene | 2016 | 5 Pages |
â¢Application of RNA interference against PLK and PNPO genes in Bombyx moriâ¢Expression of PLK and PNPO has a greater impact on transcription of PSAT and GOT.â¢PLP biosynthesis and dynamic balance may be regulated by genetic networks.
Vitamin B6 comprises six interconvertible pyridine compounds (vitamers), among which pyridoxal 5â²-phosphate is a coenzyme involved in a high diversity of biochemical reactions. Humans and animals obtain B6 vitamers from diet, and synthesize pyridoxal 5â²-phosphate by pyridoxal kinase and pyridoxine 5â²-phosphate oxidase. Currently, little is known on how pyridoxal 5â²-phosphate biosynthesis is regulated, and pyridoxal 5â²-phosphate is supplied to meet their requirement in terms of cofactor. Bombyx mori is a large silk-secreting insect, in which protein metabolism is most active, and the vitamin B6 demand is high. In this study, we successfully down-regulated the gene expression of pyridoxal kinase and pyridoxine 5â²-phosphate oxidase by body cavity injection of synthesized double-stranded small interfering RNA to 5th instar larvae of Bombyx mori, and analyzed the gene transcription levels of pyridoxal 5â²-phosphate dependent enzymes, phosphoserine aminotransferase and glutamic-oxaloacetic transaminase. Results show that the gene expression of pyridoxal kinase and pyridoxine 5â²-phosphate oxidase has a greater impact on the gene transcription of enzymes using pyridoxal 5â²-phosphate as a cofactor in Bombyx mori. Our study suggests that pyridoxal 5â²-phosphate biosynthesis and dynamic balance may be regulated by genetic networks.