Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905214 | Gene | 2016 | 8 Pages |
Abstract
Quinone reductase (QR) is an oxidative-related gene and few studies have focused on its roles concerning salt stress tolerance in plants. In this study, we cloned and analyzed the QR gene from Salix matsudana, a willow with tolerance of moderate salinity. The 612-bp cDNA corresponding to SmQR encodes 203 amino acids. Expression of SmQR in Escherichia coli cells enhanced their tolerance under salt stress. In addition, transgenic Arabidopsis thaliana lines overexpressing SmQR exhibited higher salt tolerance as compared with WT, with higher QR activity and antioxidant enzyme activity as well as higher chlorophyll content, lower methane dicarboxylic aldehyde (MDA) content and electric conductivity under salt stress. Nitro blue tetrazolium (NBT) and 3,3â²-diaminobenzidine (DAB) staining also indicated that the transgenic plants accumulated less reactive oxygen species compared to WT when exposed to salt stress. Overall, our results suggested that SmQR plays a significant role in salt tolerance and that this gene may be useful for biotechnological development of plants with improved tolerance of salinity.
Keywords
2(3)-tert-butyl-4-hydroxyanisoleNBTPVPPAPXNQO1IPTGORFBHANCBISalix matsudanaCAT1O2Na+-NQRMethane dicarboxylic aldehydeDABPODMDA3,3′-diaminobenzidineNAD(P)H:quinone oxidoreductaseO2−OH•ROSHydrogen peroxide superoxide anionSDS-PAGESinglet oxygenisopropyl β-D-1-thiogalactopyranosideOverexpressionnitro blue tetrazoliumSalt stressHydroxyl radicalSODSuperoxide dismutaseopen reading frameNational Center for Biotechnology InformationUTR یا untranslated regions untranslated regionwild-typeH2O2polymerase chain reactionPCRPeroxidaseascorbate peroxidasepolyvinylpyrrolidoneCatalaseQuinone reductaseReactive oxygen species
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Authors
Xixi Song, Jie Fang, Xiaojiao Han, Xuelian He, Mingying Liu, Jianjun Hu, Renying Zhuo,