Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905382 | Gene | 2015 | 5 Pages |
Abstract
Genetic variations in microRNAs (miRNAs) including primary miRNAs, precursor miRNAs and mature miRNAs can lead to phenotypic variation by altering the biogenesis of miRNAs and/or their binding to target mRNAs. Increasing functional studies suggest that polymorphisms occurring in miRNAs can lead to phenotypic variation in farm animal. Here, we identified a single nucleotide polymorphism (SNP) located in the precursor of chicken miRNA-1606 gene. The association study on body indexes, body weight at different growth stages, and carcass traits was performed in a Gushi-Anka F2 population resource. The SNP was not only significantly associated with body weight at 10 and 12Â weeks, respectively, but also with chicken shank length, chest depth and body slanting length at 8Â weeks; shank length, pectoral angle, body slanting length and pelvis breadth at 12Â weeks, respectively. And the polymorphism was also significantly associated with carcass traits including semi-evisceration weight, evisceration weight, breast muscle weight, leg weight and carcass weight as well. The observed values of individuals with CA genotype were significantly higher than CC genotype both in body weight at different stages and carcass traits. This SNP altered the predicted second structure of pre-mir-1606, with the altering of the free energy values. And the relative expression level of mature miRNA between CA and AA was significantly changed in leg muscle. Our data suggested that miRNA-1606 may be a candidate gene associated with chicken growth traits.
Keywords
BBLAFWprecursor miRNASewchest depthpri-miRNALMWqRT-PCRBSLGGAPrimary miRNABMWAssociation analysisPhenotypic variationPCR-RFLPChickenMicroRNAMiRNAGallus gallusquantitative real-time polymerase chain reactionbody weightCarcass weightPolymorphismspolymerase chain reaction-restriction fragment length polymorphismSingle nucleotide polymorphismpre-miRNASNP
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Hong Li, Shanhe Wang, Fengbin Yan, Xiaojun Liu, Ruirui Jiang, Ruili Han, Zhuanjian Li, Guoxi Li, Yadong Tian, Xiangtao Kang, Guirong Sun,