Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905466 | Gene | 2015 | 8 Pages |
Abstract
Peptide YY (PYY) is an anorectic brain-gut peptide involved in feeding regulation and well characterized in mammals. However, the functional role of PYY in the appetite regulatory of fish is not clear. In this study, we characterized a high conservation of PYY cDNA and found high expression levels of PYY mRNA in the brain and digestive tract of Siberian sturgeon. Then, we examined preprandial (pre- and post-feeding) changes of PYY mRNA expression in the brain that showed a significantly increased in 3Â h post-feeding, suggesting an anorectic possible function of PYY in Siberian sturgeon. Next, we examined the expression of PYY mRNA during 15Â days fasting and refeed after fasting. The SsPYY mRNA expression of unfed fish had a significant 2.4, 1.7, 2.0, 2.2, and 2.1-fold decrease compared to 1-, 3-, 6-, 10- and 15-day ad libitum fed animals, respectively. After refeed, SsPYY mRNA significantly increased 1.9 and 4.1-fold above that of the 15-day fed and unfed fish control group (PÂ <Â 0.01). Furthermore, a single intraperitoneal injection of 10, 100 and 200Â ng/g BW SsPYY1-36 caused a reduction in the next feeding and no significant reduction in food intake was observed in fish injected with a 1Â ng/g BW. Overall, PYY has a potentially role in food intake attenuation of Siberian sturgeon.
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Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Hu Chen, Xin Zhang, Jin Hao, Defang Chen, Ju Liu, Yundi Gao, Jieyao Zhu, Hongwei Wu, Fangjun Lin, Yundan Pu, Dengyue Yuan, Rongbin Wei, Chaowei Zhou, Tao Wang, Zhiqiong Li,