Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905549 | Gene | 2015 | 9 Pages |
Abstract
Cuora trifasciata has become one of the most critically endangered species in the world. The complete mitochondrial genome of C. trifasciata (Chinese three-striped box turtle) was determined in this study. Its mitochondrial genome is a 16,575-bp-long circular molecule that consists of 37 genes that are typically found in other vertebrates. And the basic characteristics of the C. trifasciata mitochondrial genome were also determined. Moreover, a comparison of C. trifasciata with Cuora cyclornata, Cuora pani and Cuora aurocapitata indicated that the four mitogenomics differed in length, codons, overlaps, 13 protein-coding genes (PCGs), ND3, rRNA genes, control region, and other aspects. Phylogenetic analysis with Bayesian inference and maximum likelihood based on 12 protein-coding genes of the genus Cuora indicated the phylogenetic position of C. trifasciata within Cuora. The phylogenetic analysis also showed that C. trifasciata from Vietnam and China formed separate monophyletic clades with different Cuora species. The results of nucleotide base compositions, protein-coding genes and phylogenetic analysis showed that C. trifasciata from these two countries may represent different Cuora species.
Keywords
Cyspicomoleadenosine 5-triphosphaterRNANADH dehydrogenasetRNACSBcytBTHRASNGLNPcGLEUALAPHEASPTrpCOXMitochondrial DNADNAnicotinamide-adenine dinucleotideATPArginineAsparagineAspartic acidalanineamino acidArgdeoxyribonucleic acidRNAribonucleic acidribosomal ribonucleic acidglutamic acidtransfer ribonucleic acidUracilisoleucineIleSERphylogenetic analysisthreonineTryptophanTyrTyrosinethymineBase pair(s)MinHISminuteMelting TemperatureSecondmtDNASubunitvalSerineHeavy cytosineCytochrome bcytochrome c oxidaseCysteinePhenylalanineLeucineMethionineMETControl regionNADHNADnanogramPROlighthistidineValinepolymerase chain reactionPCRProlineProtein Coding GeneMitochondrial genomekilobaseGluglutamineGlyGlycineGuanine
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Authors
Wei Li, Xin-Cheng Zhang, Jian Zhao, Yan Shi, Xin-Ping Zhu,