Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905585 | Gene | 2015 | 11 Pages |
Abstract
Ornamental fishes are traded with multiple names from various parts around the world, including North East India. Most are collected from the wild, due to lack of species-specific culture or breeding, and therefore, such unmanaged collection of the wild and endemic species could lead to severe threats to biodiversity. Despite many regulatory policies, trade of threatened species, including the IUCN listed species have been largely uncontrolled, due to species identification problems arising from the utilization of multiple trade names. So, the development of species-specific DNA marker is indispensable where DNA Barcoding is proved to be helpful in species identification. Here, we investigated, through DNA Barcoding and morphological assessment, the identification of 128 ornamental fish specimens exported from NE India from different exporters. The generated sequences were subjected to similarity match in BOLD-IDS as well as BLASTN, and analysed using MEGA5.2 for species identification through Neighbour-Joining (NJ) clustering, and K2P distance based approach. The analysis revealed straightforward identification of 84 specimens into 35 species, while 44 specimens were difficult to distinguish based on CO1 barcode alone. However, these cases were resolved through morphology, NJ and distanced based method and found to be belonging to 16 species. Among the 51 identified species, 14 species represented multiple trade names; 17 species belonged to threatened category. Species-level identification through DNA Barcoding along with traditional morphotaxonomy reflects its efficacy in regulating ornamental fish trade and therefore, appeals for their conservation in nature. The use of trade names rather than the zoological name created the passage for trafficking of the threatened species and demands immediate attention for sustaining wildlife conservation.
Keywords
K2PIUCNs.e.BOLDCOINumtScAMPDNAdNTPSBLAST, basic local alignment search toolInternational Union for Conservation of NatureEDTAEthylenediaminetetraacetic aciddeoxyribonucleic acidBlastDNA barcodeMolecular Evolutionary Genetic AnalysisDeoxynucleotide TriphosphatesMaximum likelihoodstandard errorCytochrome c oxidase 1Northeast IndiaOrnamental fishMEGAneighbour joiningpolymerase chain reactionPCR
Related Topics
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Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Bishal Dhar, Sankar Kumar Ghosh,