Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905790 | Gene | 2014 | 7 Pages |
Abstract
Insulin-like growth factor 2 receptor (IGF2R) is responsible for degradation of the muscle development initiator, IGF2, and thus it can be used as a marker for selection strategies in the farm animals. The aim of this study was to search for polymorphisms in three coding loci of IGF2R, and to analyze their effect on the growth traits and on the expression levels of IGF2R and IGF2 genes in the gluteus medius muscle of Egyptian buffaloes. A novel A266C SNP was detected in the coding sequences of the third IGF2R locus (at nucleotide number 51 of exon 23) among Egyptian water buffaloes. This SNP was non-synonymous mutation and led to replacement of Y (tyrosine) amino acid (aa) by D (aspartic acid) aa. Three different single-strand conformation polymorphism patterns were observed in the third IGF2R locus: AA, AC, and CC with frequencies of 0.555, 0.195, and 0.250, respectively. Statistical analysis showed that the homozygous AA genotype significantly associated with the average daily gain than AC and CC genotypes from birth to 9Â mo of age. Expression analysis showed that the A266C SNP was correlated with IGF2, but not with IGF2R, mRNA levels in the gluteus medius muscle of Egyptian buffaloes. The highest IGF2 mRNA level was estimated in the muscle of animals with the AA homozygous genotype as compared to the AC heterozygotes and CC homozygotes. We conclude that A266C SNP at nucleotide number 51 of exon 23 of the IGF2R gene is associated with the ADG during the early stages of life (from birth to 9Â mo of age) and this effect is accompanied by, and may be caused by, increased expression levels of the IGF2 gene.
Keywords
Man-6-PIGF2RIGF2SSCPHWERT-PCRSNPsADGχ2amino acideffective allele numbersEgyptian buffaloHardy–Weinberg equilibriumleast square meansreverse transcription PCRLSMinsulin-like growth factor 2Monthaverage daily gainpolymerase chain reactionPCRsingle-strand conformation polymorphismSingle nucleotide polymorphismSNPinsulin-like growth factor 2 receptor
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Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Mohammed Abu El-Magd, Haitham G. Abo-Al-Ela, Abeer El-Nahas, Ayman A. Saleh, Ali A. Mansour,