Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905799 | Gene | 2014 | 9 Pages |
Abstract
Taxus chinensis var. mairei (Taxaceae) is a domestic variety of yew species in local China. This plant is one of the sources for paclitaxel, which is a promising antineoplastic chemotherapy drugs during the last decade. We have sequenced the complete nucleotide sequence of the chloroplast (cp) genome of T. chinensis var. mairei. The T. chinensis var. mairei cp genome is 129,513Â bp in length, with 113 single copy genes and two duplicated genes (trnI-CAU, trnQ-UUG). Among the 113 single copy genes, 9 are intron-containing. Compared to other land plant cp genomes, the T. chinensis var. mairei cp genome has lost one of the large inverted repeats (IRs) found in angiosperms, fern, liverwort, and gymnosperm such as Cycas revoluta and Ginkgo biloba L. Compared to related species, the gene order of T. chinensis var. mairei has a large inversion of ~Â 110Â kb including 91 genes (from rps18 to accD) with gene contents unarranged. Repeat analysis identified 48 direct and 2 inverted repeats 30Â bp long or longer with a sequence identity greater than 90%. Repeated short segments were found in genes rps18, rps19 and clpP. Analysis also revealed 22 simple sequence repeat (SSR) loci and almost all are composed of A or T.
Keywords
Taxus chinensis var. maireiIGScpDNAPalindromicSOAPdeoxyribonucleaseSSRsSSCtRNARNaseORFlarge single-copy regionInDelLSCSSRCRACStchloroplast DNADNAseAdenosine TriphosphateATPamino acidBLAST, basic local alignment search tooltransfer ribonucleic acidBlastcoding sequenceinverted repeatinsertion/deletionsimple sequence repeatDogmaforwardribonucleaseChloroplastBLATintergenic spaceropen reading frameInvertedNADHnucleotide(s)NAD, nicotinamide adenine dinucleotidepolymerase chain reactionPCRChloroplast genomeCdSkilobasesmall single-copy region
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Authors
Yanzhen Zhang, Ji Ma, Bingxian Yang, Ruyi Li, Wei Zhu, Lianli Sun, Jingkui Tian, Lin Zhang,