Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905836 | Gene | 2014 | 8 Pages |
Abstract
Natural antisense transcripts (NATs) exist ubiquitously as pivotal molecules to regulate coding gene expression. Sirtuin 1 (Sirt1) is a NAD-dependent deacetylase which is involved in myogenesis. However, whether Sirt1 transcribes NAT during C2C12 differentiation is still unknown. In this study, we identified a Sirt1 NAT which was designated as Sirt1 antisense long non-coding RNA (AS lncRNA) by sequencing and bioinformatic analysis. The level of Sirt1 AS lncRNA was greater in spleen but less in muscle tissue. The expression of both Sirt1 mRNA and Sirt1 AS lncRNA decreased during C2C12 myogenic differentiation, whereas the levels of miR-34a, which targets Sirt1, increased gradually. We further found that the half-life of Sirt1 AS lncRNA was 10 h, but that of Sirt1 mRNA was 6 h in C2C12 cells treated with 2 μg/ml Actinomycin D. Therefore, compared with Sirt1 mRNA, Sirt1 AS lncRNA was more stable. Overexpression of Sirt1 AS lncRNA increased the levels of Sirt1 protein, whereas overexpression of Sirt1 AS lncRNA mutant did not affect the level of Sirt1 protein in C2C12 cells. Moreover, downregulation of Sirt1 mRNA caused by miR-34a was counteracted by Sirt1 AS lncRNA in C2C12 cells. Taken together, we identified a novel NAT of Sirt1 which implicated in myogenesis through regulating Sirt1 expression.
Keywords
Sirt1myogenic differentiation antigenPtprjAntisense transcriptshuman antigen RMyogeninMyoDMyHCC2C12sirtuin 1beta-site APP-cleaving enzyme 1DMEMNATBACE1lncRNAmiRNAsDulbecco's modified Eagle's mediumMiR-34aNATsRBPsDifferentiationLINE-1Natural antisense transcriptsLong noncoding RNALong interspersed element-1MYOGMicroRNARNA-binding proteinsHuR
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Authors
Yu Wang, Wei-Jun Pang, Ning Wei, Yan Xiong, Wen-Jing Wu, Cun-Zhen Zhao, Qing-Wu Shen, Gong-She Yang,