Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905885 | Gene | 2014 | 8 Pages |
Abstract
Forkhead box A2 (Foxa2) has been recognized as one of the most potent transcriptional activators that is implicated in the control of feeding behavior and energy homeostasis. However, similar researches about the effects of genetic variations of Foxa2 gene on growth traits are lacking. Therefore, this study detected Foxa2 gene polymorphisms by DNA pool sequencing, PCR-RFLP and PCR-ACRS methods in 822 individuals from three Chinese cattle breeds. The results showed that four sequence variants (SVs) were screened, including two mutations (SV1, g. 7005 C>T and SV2, g. 7044 C>G) in intron 4, one mutation (SV3, g. 8449 A>G) in exon 5 and one mutation (SV4, g. 8537 T>C) in the 3â²UTR. Notably, association analysis of the single mutations with growth traits in total individuals (at 24Â months) revealed that significant statistical difference was found in four SVs, and SV4 locus was highly significantly associated with growth traits throughout all three breeds (PÂ <Â 0.05 or PÂ <Â 0.01). Meanwhile, haplotype combination CCCCAGTC also indicated remarkably associated to better chest girth and body weight in Jiaxian Red cattle (PÂ <Â 0.05). We herein described a comprehensive study on the variability of bovine Foxa2 gene that was predictive of molecular markers in cattle breeding for the first time.
Keywords
Haplotype combinationForkhead box A2HNF-3SNPsPICHWEDlk1SVSFOXA2AssociationSequence variantsExpectation Maximizationanalysis of varianceANOVAHardy–Weinberg equilibriumfat mass and obesity associatedFTOdelta-like 1PCR–RFLPGrowth traitsHepatocyte nuclear factor 3Linkage disequilibriumpolymorphism information contentUTR یا untranslated regions Sequence variantSingle nucleotide polymorphismspolymerase chain reaction–restriction fragment length polymorphismCattle
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Authors
Mei Liu, Mijie Li, Shaoqiang Wang, Yao Xu, Xianyong Lan, Zhuanjian Li, Chuzhao Lei, Dongying Yang, Yutang Jia, Hong Chen,