Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5905987 | Gene | 2014 | 8 Pages |
Abstract
Liriodendron chinense (Hemsl.) Sarg is an endangered species and occupies a pivotal position in phylogenetic studies of flowering plants, while its genomic resources are limited. In this study, we performed transcriptome sequencing for L. chinense petals and leaves using the Illumina paired-end sequencing technique. Approximately 17.02-Gb clean reads were obtained, and de novo assembly generated 87,841 unigenes, with an average length of 778Â bp. Of these, there were 65,535 (74.61%) unigenes with significant similarity to publically available plant protein sequences. There were 3386 genes identified as significant differentially expressed between petals and leaves, among them 2969 (87.68%) were up-regulated and 417 (12.31%) down-regulated in petals. Metabolic pathway analysis revealed that 25 unigenes were predicted to be responsible for the biosynthesis of carotenoids, with 7 genes differentially expressed between these two tissues. This report is the first to identify genes associated with carotenoid biosynthesis in Liriodendron and represents a valuable resource for future genomic studies on the endangered species L. chinense.
Keywords
GGPPClusters of Orthologous Groups of proteinsUnigenesVDEZEPPSYABADEGsPDSζ-carotene desaturaseZDSCCSCoGNGSViolaxanthin de-epoxidaseCRTISOzeaxanthin epoxidaseNext-generation sequencingEndangeredphytoene synthasePhytoene desaturaseGene ontologynon-redundant protein databaseDifferentially expressed genescarotenoid isomerasegeranylgeranyl pyrophosphate synthase
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Authors
Ying Yang, Meng Xu, Qunfeng Luo, Jie Wang, Huogen Li,