Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5906005 | Gene | 2014 | 9 Pages |
Abstract
The Piwi genes have an important role in stem cell development, gametogenesis and RNA interference in diverse organisms. So far, most of the studies have focused on the function of Piwis in vertebrates, but their function during spermiogenesis in invertebrates still remains largely unclear. In order to investigate the function of Piwis during spermiogenesis in the crab Portunus trituberculatus, we use RT-PCR and RACE to identify three Piwi complete cDNA sequences from the total RNA of the testis in P. trituberculatus. The deduced amino acid sequences of P. trituberculatus Piwi-1, Piwi-2 and Piwi-3 showed that each contains a well-conserved PAZ domain and PIWI domain. RT-PCR analyzed the tissue expression pattern of P. trituberculatus Piwi-1, Piwi-2 and Piwi-3 in the testis, heart, muscle, hepatopancreas and gill. All of the Piwis are found in germ cells of adult testis in P. trituberculatus by in situ hybridization, suggesting that these genes may play function during spermiogenesis in this species.
Keywords
RT-PCRBCIPinner layerouter layerDEPCGSPPFApiRNAPortunus trituberculatusNBTPAZSSCTouchdown PCRPIWIORFUPMPBS3-aminopropyl-triethoxysilane5-bromo-4-chloro-3-indolylphosphategene specific primerAcrosomestandard saline citrateSpermiogenesisAmino acidsTestisnitroblue tetrazolium chloriderapid-amplification of cDNA endsdiethylpyrocarbonatedigoxigeninDIGPlasma membraneopen reading frameNupmembrane complexPhosphate-buffered salineApesRaceUTR یا untranslated regions untranslated regionreverse transcription-polymerase chain reactionparaformaldehydeneighbor-joiningpiwi-interacting RNA
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Genetics
Authors
Dong-Fang Xiang, Jun-Quan Zhu, Cong-Cong Hou, Wan-Xi Yang,