Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5906130 | Gene | 2013 | 6 Pages |
Abstract
As a signaling molecule, bone morphogenetic protein 8B (BMP8B) plays an essential role in bone metabolism and is able to regulate thermogenesis and energy balance, which suggests that BMP8B gene may be a new candidate for growth traits. Here, to characterize the effects of BMP8B gene on growth traits, we first used three Chinese indigenous cattle breeds (n = 845) to detect single nucleotide polymorphisms (SNPs). Five novel SNPs of BMP8B gene (g.â 242C>T, g.2164C>T, g.2639T>C, g.2900C>G and g.10817C>T) were identified by DNA pool sequencing and forced PCR-RFLP. And then we associated the five SNPs with four growth traits (body weight, body length, heart girth, and hucklebone width). Results from association analysis showed that the SNPs 1, 2, and 3 affected growth trait(s) markedly (P < 0.05). Further, 6 combined haplotypes were constructed to guarantee the reliability of analysis results. There were also significant differences in body length, heart girth and body weight between the 6 combined haplotypes (P < 0.05), but not in hucklebone width (P > 0.05). Collectively, our results suggest a modulatory role of BMP8B gene in cattle growth and development, and 3 SNPs could be used as molecular markers in early marker assisted selection (MAS) in beef cattle breeding program.
Keywords
BATTGF-βQinchuan cattlePGCTBEHWEPICGLMASPBMPsAspartic acideffective allele numbersmarker assisted selectionbrown adipose tissueMAStransforming growth factor-betaLinkage equilibriumHardy–Weinberg equilibriumBase pair(s)standard errorPCR–RFLPPrimordial germ cellGrowth traitsLinkage disequilibriumpolymorphism information contentGLM, General Linear Modelobserved heterozygosityHaplotypebone morphogenetic proteinsSNPHApCattle
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Authors
Xiu-Kai Cao, Jing Wang, Xian-Yong Lan, Chu-Zhao Lei, Chun-Lei Zhang, Xing-Lei Qi, Hong Chen,