Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5906261 | Gene | 2013 | 9 Pages |
Abstract
Toll-like receptor 3 (TLR3) plays an important role in activating innate immune responses during viral infection. In this report, TLR3 (EcTLR3) was characterized and analyzed for the first time in Epinephelus coioides. The full-length EcTLR3 cDNA is predicted to encode a 909 amino acid polypeptide that contains a signal peptide sequence, 18 leucine-rich repeat (LRR) motifs, a transmembrane region and a Toll/interleukin-1 receptor (TIR) domain. Quantitative real-time PCR revealed that the EcTLR3 mRNA was much more abundant in the liver than in other immune organs, and that the expression levels were very low in hemocyte and muscle. During development of the grouper, the levels of EcTLR3 transcripts increased with age, with very low expression levels at the early stages of development. EcTLR3 mRNA levels were examined in the liver at different times after treatment with polyriboinosinic polyribocytidylic acid (Poly I:C), and in nervous necrosis virus (NNV)-infected larval groupers. The results suggested that EcTLR3 plays an important role in a fish's defense against viral infection.
Keywords
LRRJnkPAMPsEpinephelus coioidesJun N-terminal kinaseVNNTLR3PRRsNNVIHNVTLRspathogen-associated molecular patternsInnate immunityGene expressionleucine-rich repeatTIRViral nervous necrosisInfectious hematopoietic necrosis virusnervous necrosis virusRNA virusPoly I:CToll/interleukin-1 receptorpattern recognition receptorsToll-like receptors
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Authors
Kebing Lin, Hui Ge, Qi Lin, Jianshao Wu, Libin He, Qiongshan Fang, Chen Zhou, Minqiu Sun, Zhongchi Huang,