Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5906360 | Gene | 2013 | 8 Pages |
Abstract
Drosophila Hrp38, a homolog of human hnRNP A1, has been shown to regulate splicing, but its function can be modified by poly(ADP-ribosyl)ation. Notwithstanding such findings, our understanding of the roles of poly(ADP-ribosyl)ated Hrp38 on development is limited. Here, we have demonstrated that Hrp38 is essential for fly eye development based on a rough-eye phenotype with disorganized ommatidia observed in adult escapers of the hrp38 mutant. We also observed that poly(ADP-ribose) glycohydrolase (Parg) loss-of-function, which caused increased Hrp38 poly(ADP-ribosyl)ation, also resulted in the rough-eye phenotype with disrupted ommatidial lattice and reduced number of photoreceptor cells. In addition, ectopic expression of DE-cadherin, which is required for retinal morphogenesis, fully rescued the rough-eye phenotype of the hrp38 mutant. Similarly, Parg mutant eye clones had decreased expression level of DE-cadherin with orientation defects, which is reminiscent of DE-cadherin mutant eye phenotype. Therefore, our results suggest that Hrp38 poly(ADP-ribosyl)ation controls eye pattern formation via regulation of DE-cadherin expression, a finding which has implications for understanding the pathogenic mechanisms of Hrp38-related Fragile X syndrome and PARP1-related retinal degeneration diseases.
Keywords
UASRetinitis pigmentosaGSCDE-cadherinPARP1ISESRD1PARG5′ Untranslated region5′UTRPoly(ADP-ribosyl)ationPattern formationExonic splicing enhancersEye developmentupstream activation sequenceHeterogeneous nuclear ribonucleoproteinsGermline Stem CellExonic splicing silencersDrosophilapADPrSR proteinPoly(ADP-ribose)Poly(ADP-ribose) polymerase 1DeficiencyESESpoly(ADP-ribose) glycohydrolase
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Authors
Yingbiao Ji, Michael Jarnik, Alexei V. Tulin,