Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5906543 | Gene | 2013 | 4 Pages |
Abstract
An efficient DNA assembling strategy was developed here modified from Class-IIS endonuclease mediated DNA splicing by directed ligation (SDL). Benefited from the full-length PCR directly using ligation products as template, this strategy required less effort and less time to obtain the assembled full-length DNA. The advantages of this strategy made it a rapid and easy-to-perform gene splicing and multiple site-directed mutagenesis approach especially practicable when more fragments need to be assembled at the same time.
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Authors
Lu-Bin Zhou, Qing-Qing Lin, Jing-Xian Zhang, Shu-Juan Zhao, Zhi-Bi Hu,