| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5906584 | Gene | 2013 | 9 Pages |
With the exception of target site mutations, insecticide resistance mechanisms in the principle malaria vector Anopheles gambiae, remains largely uncharacterized in Burkina Faso.Here we detected high prevalence of resistance in Vallée du Kou (VK) to pyrethroids, DDT and dieldrin, moderate level for carbamates and full susceptibility to organophosphates. High frequencies of L1014F kdr (75%) and Rdl (87%) mutations were observed showing strong correlation with pyrethroids/DDT and dieldrin resistance. The frequency of ace1R mutation was low even in carbamate resistant mosquitoes. Microarray analysis identified genes significantly over-transcribed in VK. These include the cytochrome P450 genes, CYP6P3 and CYP6Z2, previously associated with pyrethroid resistance. Gene Ontology (GO) enrichment analysis suggested that elevated neurotransmitter activity is associated with resistance, with the over-transcription of target site resistance genes such as acetylcholinesterase and the GABA receptor. A rhodopsin receptor gene previously associated with pyrethroid resistance in Culex pipiens pallens was also over-transcribed in VK.This study highlights the complex network of mechanisms conferring multiple resistance in malaria vectors and such information should be taken into account when designing and implementing resistance control strategies.
⺠High pyrethroids, DDT and dieldrin resistance in VK, moderate for carbamate ⺠High frequencies kdr and Rdl mutations in VK with strong correlation with resistance ⺠Detection using microarray of genes upregulated in VK notably CYP6P3 and CYP6Z2 P450s ⺠Neurotransmitter activity GO terms enriched in VK; Ace-1/GABA receptor upregulated ⺠A rhodopsin gene associated with resistance in Culex p. pallens upregulated in VK
