The okra (Abelmoschus esculentus) transcriptome as a source for gene sequence information and molecular markers for diversity analysis

Article ID	Journal	Published Year	Pages	File Type
5906826	Gene	2013	10 Pages	PDF

Abstract

âº RNA sequencing led to an okra transcriptome comprising >Â 150,000 unigenes. âº Unigene annotation was accomplished by sequence comparison with databases. âº 935 non-redundant SSR markers were identified in the unigene set. âº From 199 selected SSR markers 161 were validated in a germplasm panel. âº The SSR markers were applied for a diversity analysis on an okra germplasm sample.

Keywords

CTAB Abelmoschus esculentus TRIS USDA Tons dNTP SRAP UPGMA RAPD PIC CoG ORF SSR NCBI EDTA Ethylenediaminetetraacetic acid E-value EST cetyl trimethylammonium bromide Transcriptome deoxyribonucleotide triphosphate Tris(hydroxymethyl)aminomethane Next-generation sequencing Expressed Sequence Tag base pair Sra clusters of orthologous groups KEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and Genomes simple sequence repeat United States Department of Agriculture Non-redundant reduced form of nicotinamide-adenine dinucleotide open reading frame polymorphic information content National Center for Biotechnology Information NADH Microsatellite marker hectare polymerase chain reaction PCR