| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5907020 | Gene | 2012 | 9 Pages |
Abstract
⺠Serum immunoglobulins of Clarias batrachus were purified by affinity chromatography. ⺠A monoclonal antibody (E4 MAb) was produced against purified Ig of C. batrachus. ⺠E4 MAb showed binding to heavy chain of purified Ig in Western blotting. ⺠The MAb was used to quantify and demonstrate Ig + cells in blood and lymphoid organs. ⺠This MAb could detect pathogen-specific immunoglobulins in serum of immunized fish.
Keywords
TMBmAbsMNCSSCFSCFITCDMEMClarias batrachus3,3′,5,5′-tetramethyl benzidineDABPBS-T3-aminopropyltriethoxysilaneBSADulbecco's modified Eagle MediumCompetitive ELISAopdbovine serum albuminMonoclonal antibodySDS-PAGEortho-phenylenediamineimmunoglobulin immunoglobulinsELISAc-ELISAlight chainheavy chainMononuclear cellsfluorescein isothiocyanateFlow cytometryApesMolecular weightMonoclonal antibodiesforward scatterside scatteroptical densityHAT
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Authors
Neeraj Sood, Dharmendra K. Chaudhary, Akhilesh Singh, Gaurav Rathore,